6DPD
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 16 mM Mn2+ and 200 mM K+ for 40 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-BM-B |
| Synchrotron site | APS |
| Beamline | 23-BM-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.324, 37.988, 61.323 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.460 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Total number of observations | 32500 | |
| Number of reflections | 32500 | 1401 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.0 | 88.3 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
