6DOU

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 120 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8
Spacegroup name	C 1 2 1
Unit cell lengths	81.541, 37.383, 62.437
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	19.936 - 1.487
R-factor	0.1567
Rwork	0.155
R-free	0.18750
Starting model (for MR)	pdbid 1ZBL
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.940	19.940	1.510
High resolution limit [Å]	1.490	8.150	1.490
Rmerge	0.021	0.013	0.636
Rmeas	0.029	0.019	0.900
Rpim	0.021	0.013	0.636
Total number of observations	57072
Number of reflections	29596	171	1019
<I/σ(I)>	17.8
Completeness [%]	95.8	83.8	67.5
Redundancy	1.9	1.8	1.8
CC(1/2)	0.999	0.999	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2