6DOT

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8
Spacegroup name	C 1 2 1
Unit cell lengths	81.557, 37.229, 62.052
Unit cell angles	90.00, 96.61, 90.00

Refinement procedure

Resolution	23.348 - 1.423
R-factor	0.1437
Rwork	0.142
R-free	0.16840
Starting model (for MR)	1zbl
RMSD bond length	0.007
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.350	23.350	1.450
High resolution limit [Å]	1.420	7.790	1.420
Rmerge	0.033	0.028	0.422
Rmeas	0.046	0.040	0.597
Rpim	0.033	0.028	0.422
Total number of observations	67551
Number of reflections	34770	231	1527
<I/σ(I)>	11.8
Completeness [%]	99.3	96.4	87.3
Redundancy	1.9	1.7	1.9
CC(1/2)	0.998	0.995	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2