6DOR

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 300 mM K+ for 120 s at 21 C (dataset 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.544, 37.711, 62.779
Unit cell angles	90.00, 96.56, 90.00

Refinement procedure

Resolution	19.940 - 1.500
R-factor	0.1565
Rwork	0.155
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbl
RMSD bond length	0.009
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.940	19.940	1.520
High resolution limit [Å]	1.500	8.200	1.500
Total number of observations	30511
Number of reflections	30511	176	1322
<I/σ(I)>	8
Completeness [%]	99.3	85.9	88.7
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride