6DOJ
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 5 mM K+ for 120 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.873, 37.361, 62.275 |
Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
Resolution | 20.018 - 1.403 |
R-factor | 0.1469 |
Rwork | 0.145 |
R-free | 0.18460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zbl |
RMSD bond length | 0.007 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.020 | 20.020 | 1.430 |
High resolution limit [Å] | 1.400 | 7.680 | 1.400 |
Total number of observations | 36467 | ||
Number of reflections | 36467 | 223 | 1473 |
<I/σ(I)> | 5.8 | ||
Completeness [%] | 98.9 | 89.1 | 81.6 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride |