6DNQ

HBZ77 in complex with KIX and c-Myb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	118.825, 51.166, 72.047
Unit cell angles	90.00, 126.41, 90.00

Refinement procedure

Resolution	36.019 - 2.350
R-factor	0.2001
Rwork	0.199
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2agh
RMSD bond length	0.004
RMSD bond angle	0.721
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.120
Rmeas	0.130
Rpim	0.060
Number of reflections	14320
<I/σ(I)>	13.7
Completeness [%]	97.1
Redundancy	4.5
CC(1/2)	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, calcium chloride