6DN6
SPRY domain-containing SOCS box protein 2 complexed with INNN(ABU) cyclic peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.040, 61.950, 84.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.460 - 1.590 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.074 | 0.743 |
| Rpim | 0.024 | 0.241 |
| Number of reflections | 29881 | |
| <I/σ(I)> | 22.6 | 3.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277.15 | 0.1M CHES, 20% PEG 8,000 |
