6DMX
HBZ56 in complex with KIX and c-Myb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.997, 80.311, 64.641 |
Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
Resolution | 40.970 - 2.800 |
R-factor | 0.24198 |
Rwork | 0.239 |
R-free | 0.30083 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2agh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.056 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 13196 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 95.2 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, ammonium iodide |