6DLP
Crystal structure of LRRK2 WD40 domain dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.89260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.293, 103.590, 112.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.256 - 4.000 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.26330 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.660 | 3.780 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 12211 | |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 95.8 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Tris-HCl at pH 8.5, 1 M LiCl, 16% polyethylene glycol (PEG) 6000, and 10% additive of 30% galactose. The crystal was soaked in 1 mM trans-platinum (II) diammine dichloride for 1.5 hours |
