6DKA
Yeast Ddi2 Cyanamide Hydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0246 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 263.430, 263.430, 119.214 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.966 - 2.901 |
| R-factor | 0.1986 |
| Rwork | 0.198 |
| R-free | 0.22960 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6dk9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.114 | 0.630 |
| Number of reflections | 101420 | 5107 |
| <I/σ(I)> | 16.1 | 2.2 |
| Completeness [%] | 96.8 | 98.4 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 1.1-1.3 M Ammonium Sulfate 0.2 M Arginine 0.1 M N-morpholino ethane sulfonate pH 5.8 |
