6DK8
RetS kinase region without cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9739 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 158.708, 158.708, 243.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.779 - 3.800 |
| R-factor | 0.2155 |
| Rwork | 0.215 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dk7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.754 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.780 | 3.936 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 31211 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 26.2 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 2.7 M sodium chloride, 0.1 M HEPES, pH 7.5 |
