6DJ9

Structure of the USP15 DUSP domain in complex with a high-affinity Ubiquitin Variant (UbV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 63
Unit cell lengths	152.410, 152.410, 125.461
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	90.935 - 3.100
R-factor	0.2548
Rwork	0.253
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 4A3O
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.935	3.090
High resolution limit [Å]	2.930	2.930
Rmerge	0.102	1.463
Rmeas	0.113	1.621
Rpim	0.049	0.694
Number of reflections	69830	10150
<I/σ(I)>	8.8	1.2
Completeness [%]	100.0	100
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	16% PEG6K, 100 mM Sodium Citrate, pH 5.0, 100 mM MgCl2, cryoprotected in 20% PEG6K, 100 mM Sodium Citrate, pH 5.0, 100 mM MgCl2, 30% ethylene glycol