6DJ3

Crystal structure of CNNM2 cyclic nucleotide-binding homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-22
Detector	RAYONIX MX-300
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	110.582, 110.582, 84.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.900 - 2.600
Rwork	0.219
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dfd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12-2829)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	14399	318
<I/σ(I)>	19.8	0.8
Completeness [%]	96.5	70.9
Redundancy	8.7	5.4
CC(1/2)		0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M Bis-Tris pH 6.0, 0.5 M Na Citrate pH 7.0