6DII
Structure of Arabidopsis Fatty Acid Amide Hydrolase in Complex with methyl linolenyl fluorophosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Collection date | 2017-05-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 225.577, 83.311, 272.811 |
| Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
| Resolution | 34.614 - 3.200 |
| R-factor | 0.2547 |
| Rwork | 0.253 |
| R-free | 0.29610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dhv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
| Rmerge | 0.108 | 0.042 | 0.543 |
| Rmeas | 0.132 | 0.050 | 0.682 |
| Rpim | 0.075 | 0.027 | 0.405 |
| Total number of observations | 368215 | ||
| Number of reflections | 132397 | 16171 | 10900 |
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 83.0 | 98.4 | 69.1 |
| Redundancy | 2.8 | 3.3 | 2.3 |
| CC(1/2) | 0.998 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 0.1 M MES, pH 6.0, 30% PEG 200, 5% PEG 3350 |
