6DGK

Crystal Structure of the Non-Structural Protein 1 (NS1) effector domain W187A mutant from the A/Brevig Mission/1/1918 (H1N1) strain of Influenza A Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-14
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	32.785, 69.744, 104.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 1.900
R-factor	0.173
Rwork	0.170
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.247
Rmeas	0.065	0.266
Rpim	0.025	0.099
Number of reflections	19479	1905
<I/σ(I)>	54.9	8.99
Completeness [%]	98.7	98.45
Redundancy	7.1
CC(1/2)	0.992	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	30% PEG 3350, 0.2M di-Ammonium tartrate