6DG4

Structure of the Chaetomium thermophilum Ulp1-like SUMO protease catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	45.986, 63.595, 83.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.842 - 1.442
R-factor	0.1195
Rwork	0.118
R-free	0.14810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1euv
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.460
High resolution limit [Å]	1.440	3.910	1.440
Rmerge	0.052	0.039	0.215
Rmeas	0.056	0.043	0.234
Rpim	0.021	0.017	0.091
Total number of observations	310342
Number of reflections	44305	2401	1878
<I/σ(I)>	9.4
Completeness [%]	97.9	97.2	85.2
Redundancy	7	6.6	6.2
CC(1/2)		0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES, pH 7.0, 2.1 M ammonium sulfate