6DG2

Antigen Binding Fragment of the Pan-ebolavirus Monoclonal Antibody 6D6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	70.226, 72.038, 107.149
Unit cell angles	90.00, 104.20, 90.00

Refinement procedure

Resolution	68.080 - 1.960
R-factor	0.1922
Rwork	0.191
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i9r
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.040	2.000
High resolution limit [Å]	1.960	1.960
Rmerge	0.070	1.000
Number of reflections	70193
<I/σ(I)>	7.6	1.3
Completeness [%]	94.2	98.8
Redundancy	3.3	3.3
CC(1/2)	0.990	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1 M Tris pH 7.6, 25% w/v PEG 6000