6DFS

mouse TCR I.29 in complex with IAg7-p8E9E6ss

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 63
Unit cell lengths	269.518, 269.518, 45.639
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 3.100
R-factor	0.24197
Rwork	0.240
R-free	0.28515
RMSD bond length	0.017
RMSD bond angle	2.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.100	3.100
Number of reflections	32259
<I/σ(I)>	24.4
Completeness [%]	99.7
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% PEG 20000, 100mM bicine pH9.0, 20% ethylene glycol