6DFK
Crystal structure of the 11S subunit of the Plasmodium falciparum proteasome, PA28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 166.485, 166.485, 399.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.890 - 3.100 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.636 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.305 | |
| Rpim | 0.131 | |
| Number of reflections | 116975 | 5711 |
| <I/σ(I)> | 7.5 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.7 |
| CC(1/2) | 0.996 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20 mM HEPES, 2 M ammonium sulfate |
