6DFK

Crystal structure of the 11S subunit of the Plasmodium falciparum proteasome, PA28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	166.485, 166.485, 399.162
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.890 - 3.100
R-factor	0.183
Rwork	0.182
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1avo
RMSD bond length	0.003
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	3.150
High resolution limit [Å]	3.100	3.100
Rmeas	0.305
Rpim	0.131
Number of reflections	116975	5711
<I/σ(I)>	7.5	1.2
Completeness [%]	100.0	100
Redundancy	10.4	10.7
CC(1/2)	0.996	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20 mM HEPES, 2 M ammonium sulfate