6DFB

Kaiso (ZBTB33) K539A zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 2
Unit cell lengths	44.467, 184.271, 105.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.198 - 1.660
R-factor	0.1811
Rwork	0.179
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.009
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.198	1.720
High resolution limit [Å]	1.660	1.660
Rmerge	0.074
Rmeas	0.077
Number of reflections	50786	4731
<I/σ(I)>	14.89	2.83
Completeness [%]	98.3	92.65
Redundancy	11.2	7.9
CC(1/2)	0.997	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350