6DF2
Improved anti-phosphotyrosine antibody 4G10-S5-4D5 Fab complexed with phosphotyrosine peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.510, 110.550, 69.080 |
Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
Resolution | 83.656 - 2.600 |
R-factor | 0.1793 |
Rwork | 0.177 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bj1 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.767 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.000 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.210 | 1.258 |
Number of reflections | 29644 | 2940 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 26% PEG3350, 0.1M Bis-Tris, and 0.16M ammonium acetate |