6DF0

anti-phosphotyrosine antibody 4G10-4D5 Fab complexed with sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11583
Spacegroup name	P 61 2 2
Unit cell lengths	98.900, 98.900, 207.364
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.650 - 2.300
R-factor	0.2024
Rwork	0.200
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bj1
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.650	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.056	0.695
Rmeas	0.072	0.715
Rpim	0.017	0.168
Number of reflections	27809	3136
<I/σ(I)>	22.7
Completeness [%]	100.0	100
Redundancy	17.6	18
CC(1/2)	1.000	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG4000, 0.2M ammonium sulfate, and 0.1M sodium acetate