6DEG

Crystal structure of a DNA polymerase III subunit beta DnaN sliding clamp from Bartonella birtlesii LL-WM9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97982
Spacegroup name	P 1 21 1
Unit cell lengths	53.510, 157.670, 53.540
Unit cell angles	90.00, 110.81, 90.00

Refinement procedure

Resolution	47.702 - 2.450
R-factor	0.2189
Rwork	0.215
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.702	47.702	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.040	0.019	0.549
Rmeas	0.046	0.022	0.636
Total number of observations	114643
Number of reflections	29973	334	2204
<I/σ(I)>	19.48	49.01	2.27
Completeness [%]	98.2	92.5	98.9
Redundancy	3.825	3.527	3.906
CC(1/2)	0.999	0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	287	BabiA.17987.a.B1.PS38344 at 13.7 mg/mL against JCSG+ screen condition H7 0.2 M ammonium sulfate, 0.1 M BisTris pH 5.5, 25% PEG 3350, 15% EG as cryo-protectant, crystal tracking ID 295132h7, unique puck ID zts9-10