6DEF
Vps1 GTPase-BSE fusion complexed with GMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.659, 118.740, 84.140 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 40.786 - 2.260 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.790 | 2.310 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.068 | 0.445 |
| Rmeas | 0.080 | 0.590 |
| Rpim | 0.042 | 0.316 |
| Number of reflections | 69139 | 4347 |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 92.4 | 90.5 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.997 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG4000, 0.2 M sodium acetate, 0.1 M Tris-Cl, pH 8.7-9.0 |
