6DE8
Crystal Structure of Bifunctional Enzyme FolD-Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97901 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 164.887, 59.253, 73.240 |
Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
Resolution | 42.770 - 2.104 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22590 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.465 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.122 | 0.881 |
Number of reflections | 40403 | 2008 |
<I/σ(I)> | 14.4 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.5 | 4.4 |
CC(1/2) | 0.651 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M potassium iodide, 20 % (v/v) PEG 3350 |