6DD9
Structure of mouse SYCP3, P1 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 |
Unit cell lengths | 45.869, 52.275, 75.595 |
Unit cell angles | 95.17, 103.70, 110.54 |
Refinement procedure
Resolution | 72.103 - 2.300 |
R-factor | 0.2894 |
Rwork | 0.287 |
R-free | 0.33100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dd8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.933 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.110 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.088 | 0.967 |
Number of reflections | 51353 | 8372 |
<I/σ(I)> | 9.98 | 1.45 |
Completeness [%] | 92.0 | 92.6 |
Redundancy | 3.7 | 3.7 |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-HCl pH 8.5, 16% PEG 4000, 100-200 mM sodium acetate |