6DCV

Crystal structure of human anti-tau antibody CBTAU-27.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0330
Spacegroup name	C 1 2 1
Unit cell lengths	93.411, 60.578, 169.472
Unit cell angles	90.00, 103.52, 90.00

Refinement procedure

Resolution	46.533 - 1.900
R-factor	0.1881
Rwork	0.186
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3QOS
RMSD bond length	0.007
RMSD bond angle	1.169
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rpim	0.050	0.480
Number of reflections	72579
<I/σ(I)>	17.3	1.5
Completeness [%]	99.3
Redundancy	5.7
CC(1/2)	0.998	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes, pH 7.5, 20% PEG8000