6DCS
Stage III sporulation protein AF (SpoIIIAF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.286 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 119.365, 119.365, 40.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.659 - 2.700 |
R-factor | 0.2431 |
Rwork | 0.240 |
R-free | 0.29940 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.238 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.084 | 0.057 | 0.304 |
Rmeas | 0.091 | 0.062 | 0.345 |
Rpim | 0.034 | 0.024 | 0.157 |
Total number of observations | 56622 | ||
Number of reflections | 8421 | 508 | 332 |
<I/σ(I)> | 11.6 | ||
Completeness [%] | 98.0 | 99.8 | 80 |
Redundancy | 6.7 | 6.4 | 3.7 |
CC(1/2) | 0.997 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2M Ammonium sulfate |