6DCM
The atomic resolution crystal structure of Kringle 2 variant bound with EACA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.825, 39.006, 31.316 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 23.458 - 1.031 |
| R-factor | 0.1423 |
| Rwork | 0.141 |
| R-free | 0.15690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i5k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.460 | 1.068 |
| High resolution limit [Å] | 1.031 | 1.031 |
| Rmerge | 0.062 | 0.368 |
| Rmeas | 0.070 | 0.447 |
| Number of reflections | 31093 | |
| <I/σ(I)> | 34.5 | 4.7 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | PEG 4000, Sodium acetate, Ammonium acetate |
