6DC0

Tribbles (TRIB1) pseudokinase fused to CCAAT-enhancer binding protein (C/EBPalpha) degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	CCD
Collection date	2016-02-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	98.809, 98.809, 332.705
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.700 - 2.800
R-factor	0.22254
Rwork	0.220
R-free	0.27679
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cem
RMSD bond length	0.011
RMSD bond angle	1.654
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	24777
<I/σ(I)>	19.8
Completeness [%]	99.4
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2.8 M Na Acetate, 0.1 M BIS-TRIS prop 7 pH with 5% sodium citrate