6DB6

Crystal structure of anti-HIV-1 V3 Fab 311-11D in complex with a HIV-1 gp120 V3 peptide from MN strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 21 21
Unit cell lengths	46.568, 69.157, 141.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.218 - 1.978
R-factor	0.1761
Rwork	0.173
R-free	0.21640
RMSD bond length	0.007
RMSD bond angle	0.931
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.220	2.049
High resolution limit [Å]	1.978	1.978
Rmerge	0.117	0.784
Number of reflections	32451
<I/σ(I)>	10.1
Completeness [%]	98.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1 M Imidazole pH 6.5, 15% PEG 8000, 30% Isopropanol