6DAF

2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	34.355, 121.309, 125.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.678 - 2.400
R-factor	0.205
Rwork	0.203
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2be6
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.678	43.678	2.550
High resolution limit [Å]	2.400	7.120	2.400
Rmeas	0.057		0.523
Number of reflections	39395		6345
<I/σ(I)>	13.31		2.43
Completeness [%]	99.1		98.8
Redundancy	3.09
CC(1/2)	0.998		0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.5 M potassium nitrate, 20% PEG3350