6D9K
Ternary RsAgo Complex with Guide RNA and Target DNA Containing A-G Non-canonical Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.119, 119.130, 118.134 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 43.540 - 2.000 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.613 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.591 |
| Number of reflections | 123233 | |
| <I/σ(I)> | 14.9 | 1.8 |
| Completeness [%] | 97.4 | 90.4 |
| Redundancy | 3.8 | 2.6 |
| CC(1/2) | 0.991 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |
