6D9G
X-ray Structure of the FAB Fragment of 15B8, a Murine Monoclonal Antibody Specific for the Human Serotonin Transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 82.870, 85.490, 141.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.300 |
R-factor | 0.1965 |
Rwork | 0.195 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i66 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.595 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2597) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.930 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.092 | 0.413 |
Number of reflections | 45074 | 4199 |
<I/σ(I)> | 17.88 | 2.68 |
Completeness [%] | 99.1 | 94.2 |
Redundancy | 10.8 | 4.6 |
CC(1/2) | 0.999 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.2 M ammonium sulfate, 0.08 M sodium citrate pH 5.2 |