6D7J
The Crystal Structure of Parabacteroides merdae Beta-Glucuronidase (GUS) with Glycerol in Active-Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.920, 171.968, 125.322 |
Unit cell angles | 90.00, 107.84, 90.00 |
Refinement procedure
Resolution | 48.340 - 2.240 |
R-factor | 0.1653 |
Rwork | 0.165 |
R-free | 0.20020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.889 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.340 | 2.325 |
High resolution limit [Å] | 2.240 | 2.244 |
Number of reflections | 335371 | |
<I/σ(I)> | 9.64 | |
Completeness [%] | 96.2 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 10% PEG 3350 0.2 M Proline 0.1 M HEPES pH 7.4 |