6D7A

Structure of T. gondii PLP1 beta-rich domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-25
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	101.980, 50.850, 105.340
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	24.883 - 1.130
R-factor	0.1418
Rwork	0.141
R-free	0.16370
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.044
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.883	1.170
High resolution limit [Å]	1.130	1.130
Rmerge	0.077
Rmeas	0.084
Rpim	0.034
Number of reflections	191300
<I/σ(I)>	7.99
Completeness [%]	94.9
Redundancy	4.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	100 mM sodium cacodylate, 0.8M sodium formate, 15% PEG 4000