6D6G

Triclinic lysozyme (295 K) in the presence of 47% MPD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Agilent SuperNova
Temperature [K]	295
Detector technology	CCD
Collection date	2016-01-20
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	27.333, 32.151, 34.318
Unit cell angles	88.24, 70.78, 68.69

Refinement procedure

Resolution	13.613 - 2.000
R-factor	0.1337
Rwork	0.132
R-free	0.18190
RMSD bond length	0.002
RMSD bond angle	0.501
Data scaling software	SCALA
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.620	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.072	0.278
Number of reflections	6714
<I/σ(I)>	13.1	4.4
Completeness [%]	97.3
Redundancy	2.3
CC(1/2)	0.997	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein: 10 mg/mL in water Well: 0.3 M NaNO3