6D5Y

Crystal structure of ERK2 G169D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	93
Detector technology	CCD
Collection date	2011-01-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.03317
Spacegroup name	P 21 21 21
Unit cell lengths	44.870, 65.400, 116.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.860
R-factor	0.2218
Rwork	0.220
R-free	0.27190
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.860	2.860
Number of reflections	57377
<I/σ(I)>	18.5
Completeness [%]	92.3
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% PEG 3350, 10% isopropanol, and 0.1 M Hepes pH 7.5