6D5W
Ras:SOS:Ras in complex with a small molecule activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 186.125, 186.125, 179.217 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.033 - 2.478 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nyi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.082 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 |
High resolution limit [Å] | 2.470 | 2.470 |
Number of reflections | 54650 | 2718 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 99.4 | |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0 |