6D5J

Ras:SOS:Ras in complex with a small molecule activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
Unit cell lengths	183.627, 183.627, 179.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.718 - 1.751
R-factor	0.1576
Rwork	0.157
R-free	0.17500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nyi
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((dev_2875: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rpim	0.027	0.248
Number of reflections	151885	7511
<I/σ(I)>	26.9
Completeness [%]	100.0
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0