6D56
Ras:SOS:Ras in complex with a small molecule activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 183.621, 183.621, 178.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.213 - 1.680 |
R-factor | 0.1595 |
Rwork | 0.159 |
R-free | 0.17070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nyi |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2875: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.273 | |
Rpim | 0.026 | |
Number of reflections | 171953 | 8516 |
<I/σ(I)> | 28.7 | |
Completeness [%] | 100.0 | |
Redundancy | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0 |