6D4O
Eubacterium eligens beta-glucuronidase bound to an amoxapine-glucuronide conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 179.954, 179.954, 134.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.450 - 2.900 |
| R-factor | 0.2023 |
| Rwork | 0.199 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.293 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.147 | 0.498 |
| Rmeas | 0.155 | 0.523 |
| Number of reflections | 29069 | 4621 |
| <I/σ(I)> | 17 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.3 | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.05 M Bis-tris pH 6.5 45% PEG 400 |
