6D4N

Crystal structure of a Fc fragment of Rhesus macaque (Macaca mulatta) IgG4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 32
Unit cell lengths	66.552, 66.552, 90.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.634 - 3.250
R-factor	0.2148
Rwork	0.213
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c54
RMSD bond length	0.008
RMSD bond angle	1.264
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.250	3.250
Rmerge	0.090	0.971
Rpim	0.044	0.491
Number of reflections	7018
<I/σ(I)>	31.3	1.2
Completeness [%]	98.9	99.7
Redundancy	5.1	4.8
CC(1/2)	0.990	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	1.34% PEG 8000 335 ammonium citrate pH 8.5