6D4I

Crystal Structure of a Fc Fragment of Rhesus macaque (Macaca mulatta) IgG2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 32
Unit cell lengths	66.916, 66.916, 87.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.905 - 2.950
R-factor	0.2095
Rwork	0.207
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4haf
RMSD bond length	0.008
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.087	0.657
Rpim	0.064	0.485
Number of reflections	8919
<I/σ(I)>	13.6	1.3
Completeness [%]	96.1	97.7
Redundancy	2.6	2.7
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	3 M sodium chloride 0.1 M sodium acetate pH 4.5