6D4G

N-GTPase domain of p190RhoGAP-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	43.690, 69.270, 154.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.032 - 2.800
R-factor	0.252
Rwork	0.250
R-free	0.29010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c5h
RMSD bond length	0.008
RMSD bond angle	0.663
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.032	42.032	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.179	0.085	3.529
Rmeas	0.181	0.086	3.561
Total number of observations	624368
Number of reflections	12101	1307	1192
<I/σ(I)>	20.41	48.85	1.87
Completeness [%]	99.8	98.6	100
Redundancy	51.596	45.59	54.501
CC(1/2)	0.999	0.999	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	25% (w/v) PEG 8000, 100 mM Tris pH 8.5