6D48
Cell Surface Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.801, 41.543, 107.498 |
| Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
| Resolution | 35.599 - 1.776 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nko |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1496: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.480 | 1.810 |
| High resolution limit [Å] | 1.776 | 1.776 |
| Rmerge | 0.044 | 0.391 |
| Rmeas | 0.052 | 0.464 |
| Rpim | 0.028 | 0.246 |
| Number of reflections | 51251 | 2673 |
| <I/σ(I)> | 14.7 | 3.1 |
| Completeness [%] | 98.5 | 90.3 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 4000 100 mM Tris-HCl pH 8.5 10% Glycerol |
