6D46

Crystal structure of a DNA polymerase III subunit beta DnaN sliding clamp from Rickettsia typhi str. Wilmington

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	147.520, 43.050, 83.170
Unit cell angles	90.00, 121.39, 90.00

Refinement procedure

Resolution	41.533 - 2.000
R-factor	0.1771
Rwork	0.174
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w7z
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.533	41.533	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.049	0.027	0.489
Rmeas	0.056	0.032	0.559
Number of reflections	30248	370	2239
<I/σ(I)>	16	34.14	3.52
Completeness [%]	98.7	96.1	98.4
Redundancy	4.118	3.514	4.228
CC(1/2)	0.999	0.998	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	287	24.49 mg/mL RityA.17987.a.B1.PW38354 against JCSG+ screen condition C6 = 0.1 M phosphate/citrate pH 4.2, 40% PEG 300, direct cryo, 295697c6, ffq4-2