6D31

Structure of human Usb1 with adenosine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.430, 52.077, 46.240
Unit cell angles	90.00, 106.26, 90.00

Refinement procedure

Resolution	44.390 - 1.200
R-factor	0.149
Rwork	0.147
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v1m
RMSD bond length	0.022
RMSD bond angle	1.676
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	57826
<I/σ(I)>	12.5
Completeness [%]	97.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	100 mM bis-tris, 25% SOKALAN CP 42, 0.9% tetrahydrofuran