6D11
Crystal structure of 1450 Fab in complex with circumsporozoite protein NANP5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.620, 135.058, 344.092 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.490 - 3.400 |
| R-factor | 0.2563 |
| Rwork | 0.254 |
| R-free | 0.29930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.692 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.311 | 0.727 |
| Rpim | 0.165 | 0.386 |
| Number of reflections | 15950 | 2452 |
| <I/σ(I)> | 4.5 | 1.3 |
| Completeness [%] | 92.1 | 92.9 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.957 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 22.5% (w/v) PEG3350, 0.2M di-ammonium hydrogen citrate |
