6D0Z

Polymerase Eta cytarabine (AraC) extension ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	98.789, 98.789, 81.772
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.113 - 1.750
R-factor	0.1697
Rwork	0.168
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mr2
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.550	1.710
High resolution limit [Å]	1.680	1.680
Number of reflections	858160	43481
<I/σ(I)>	17.5	0.5
Completeness [%]	100.0	100
Redundancy	16.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MES buffer, PEG1500, magnesium chloride